Dinucleotide repeat polymorphism located at D4S169
نویسندگان
چکیده
منابع مشابه
Dinucleotide repeat polymorphism at the IGF2R locus.
References: 1) Oshima,A. et al. (1988) J. Biol. Chem. 263, 2553-2562. 2) Laureys.G. etal. (1988) Genomicsi, 224-229. 2/162 6/162 129/162 20/162 1/162 4/162 0.01 0.04 0.80 0.12 0.01 0.02 Source/Description: The clone VAW140 (1) was tested for CA repeats by Southern blot hybridization (2). One CA-hybridizing Hindm fragment was subcloned into mp8 in both orientations and sequenced at each end. A (...
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PCR Conditions: PCR reactions are carried out in a total volume of 25 fi\ containing: 25 ng genomic DNA, 5 pmole of each primer, 1.5 mM MgCl2, 200 jiM dNTPs, 50 mM tris-Cl, 20 mM (NH^SO,,, pH 9, 0.25 mM spermidine and 0.6 units of ReplinaseTM (NEN/Dupont). Amplification was performed in 96 well plates in a BiosyclerTM oven (Bios Corp., New Haven, CT) for 32 cycles. We used a 4 temperature cycle...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1991
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/19.22.6347-a